Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
Identifieur interne : 000121 ( Main/Exploration ); précédent : 000120; suivant : 000122Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
Auteurs : Zhizhen Fang ; Chengchun Lai ; Yaling Zhang ; Zhongxiong LaiSource :
- SpringerPlus [ 2193-1801 ] ; 2016.
Abstract
To clone and examine expression profiles of
The online version of this article (doi:10.1186/s40064-016-1887-0) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s40064-016-1887-0
PubMed: 27026877
PubMed Central: 4766155
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning, structural and expression profiling of <italic>DlRan</italic> genes during somatic embryogenesis in <italic>Dimocarpus longan</italic> Lour.</title><author><name sortKey="Fang, Zhizhen" sort="Fang, Zhizhen" uniqKey="Fang Z" first="Zhizhen" last="Fang">Zhizhen Fang</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Lai, Chengchun" sort="Lai, Chengchun" uniqKey="Lai C" first="Chengchun" last="Lai">Chengchun Lai</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Yaling" sort="Zhang, Yaling" uniqKey="Zhang Y" first="Yaling" last="Zhang">Yaling Zhang</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Lai, Zhongxiong" sort="Lai, Zhongxiong" uniqKey="Lai Z" first="Zhongxiong" last="Lai">Zhongxiong Lai</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27026877</idno><idno type="pmc">4766155</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766155</idno><idno type="RBID">PMC:4766155</idno><idno type="doi">10.1186/s40064-016-1887-0</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000700</idno><idno type="wicri:Area/Pmc/Curation">000699</idno><idno type="wicri:Area/Pmc/Checkpoint">000100</idno><idno type="wicri:Area/Ncbi/Merge">001F75</idno><idno type="wicri:Area/Ncbi/Curation">001F75</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F75</idno><idno type="wicri:Area/Main/Merge">000121</idno><idno type="wicri:Area/Main/Curation">000121</idno><idno type="wicri:Area/Main/Exploration">000121</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular cloning, structural and expression profiling of <italic>DlRan</italic> genes during somatic embryogenesis in <italic>Dimocarpus longan</italic> Lour.</title><author><name sortKey="Fang, Zhizhen" sort="Fang, Zhizhen" uniqKey="Fang Z" first="Zhizhen" last="Fang">Zhizhen Fang</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Lai, Chengchun" sort="Lai, Chengchun" uniqKey="Lai C" first="Chengchun" last="Lai">Chengchun Lai</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Yaling" sort="Zhang, Yaling" uniqKey="Zhang Y" first="Yaling" last="Zhang">Yaling Zhang</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author><author><name sortKey="Lai, Zhongxiong" sort="Lai, Zhongxiong" uniqKey="Lai Z" first="Zhongxiong" last="Lai">Zhongxiong Lai</name><affiliation><nlm:aff id="Aff1">Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou, 350002 Fujian China</nlm:aff><wicri:noCountry code="subfield">350002 Fujian China</wicri:noCountry></affiliation></author></analytic><series><title level="j">SpringerPlus</title><idno type="eISSN">2193-1801</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To clone and examine expression profiles of <italic>DlRan</italic> genes during somatic embryogenesis in <italic>Dimocarpus longan</italic> Lour. Thirty cDNA sequences and two genomic sequences encoding DlRan proteins were isolated from longan embryogenic cultures. Structural analysis of <italic>DlRan</italic> genes revealed that the longan <italic>Ran</italic> gene family is more expanded than that of Arabidopsis. Expression analysis of <italic>DlRan</italic> genes during somatic embryogenesis uncovered a high abundance of <italic>DlRan</italic> genes in early embryogenic cultures and heart- and torpedo-shaped embryos. The expression of <italic>DlRan</italic> genes in embryogenic calli was affected by exogenous 2,4-dichlorophenoxyacetic acid treatment. <italic>DlRan</italic> is involved in 2,4-D induced somatic embryogenesis and development of somatic embryos in longan.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s40064-016-1887-0) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aiqing, J" uniqKey="Aiqing J">J Aiqing</name></author><author><name sortKey="Xueqing, G" uniqKey="Xueqing G">G Xueqing</name></author><author><name sortKey="Yan, Z" uniqKey="Yan Z">Z Yan</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H Yang</name></author><author><name sortKey="Guoliang, W" uniqKey="Guoliang W">W Guoliang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Cl" 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uniqKey="Xie H">HH Xie</name></author><author><name sortKey="Jiang, Ym" uniqKey="Jiang Y">YM Jiang</name></author><author><name sortKey="Chen, F" uniqKey="Chen F">F Chen</name></author><author><name sortKey="Wei, Xy" uniqKey="Wei X">XY Wei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zuker, M" uniqKey="Zuker M">M Zuker</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Fang, Zhizhen" sort="Fang, Zhizhen" uniqKey="Fang Z" first="Zhizhen" last="Fang">Zhizhen Fang</name><name sortKey="Lai, Chengchun" sort="Lai, Chengchun" uniqKey="Lai C" first="Chengchun" last="Lai">Chengchun Lai</name><name sortKey="Lai, Zhongxiong" sort="Lai, Zhongxiong" uniqKey="Lai Z" first="Zhongxiong" last="Lai">Zhongxiong Lai</name><name sortKey="Zhang, Yaling" sort="Zhang, Yaling" uniqKey="Zhang Y" first="Yaling" last="Zhang">Yaling Zhang</name></noCountry></tree></affiliations></record>Pour 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